Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Delivery of immunoglobulin G antibodies to the rat nervous system following intranasal administration: Distribution, dose-response, and mechanisms of delivery

doi: 10.1016/j.jconrel.2018.08.006

Figure Lengend Snippet: Approximate nervous system regions dissected for gamma counting to measure [125I]-IgG concentration. Red dashed lines indicate microdissected regions of interest from saline perfused and aldehyde fixed rats following intranasal or intra-arterial [125I]-IgG dosing. (A) The olfactory bulbs, midbrain, pons, medulla, cerebellum, and cervical spinal cord were sampled as gross anatomical regions. A1 to A9 are representative schema for 1 mm serial coronal brain sections made using a coronal precision rat brain matrix (Brain Tree Scientific Inc.) as shown in A. A scalpel (# 10 blade – Royal Tek) or 1–2 mm sample corers (Fine Science Tools) were used to dissect specific brain regions from each of the serial coronal brain sections using a micropunch technique [39]. All brain dissections were performed with tissue visualized under a stereo microscope (Olympus SZ61). Brain regions were dissected from the coronal tissue sections as follows - the frontal cortex from slices A1 and A2, motor cortex from slices A2 - A9, primary somatosensory cortex from slices A3 – A9, caudoputamen from slices A4 – A6, and hippocampus from slices A8 and A9. (B) Schematic of the base of the skull showing trigeminal nerve components. The ophthalmic (V1) and maxillary (V2) segments of the trigeminal nerve, extending from the anterior lacerated foramen to the point where the nerve root contacts the brainstem, were dissected for gamma counting separately from the mandibular segment (V3). (C) Anterior circulation (anterior and middle cerebral arteries) and posterior circulation (basilar and vertebral arteries) were marked with India ink and then separately dissected by making cuts at the left and right posterior communicating arteries using Vannas scissors (Harvard Apparatus). Asterisk denotes cut internal carotid arteries. Abbreviations: olf. bulb – olfactory bulb; rf – rhinal fissure; cer. sp. cord – cervical spinal cord; fr. ctx – frontal cortex; mtr. ctx – motor cortex; S1 ctx – primary somatosensory cortex; CPu – caudoputamen; ac – anterior commissure; hipp. – hippocampus; ant. lac. for. – anterior lacerated foramen; for. ov. – foramen ovale; trig. nerve – trigeminal nerve; trig. art. – trigeminal artery; for. – foramen; np – nasal passage; V1 – ophthalmic branch of the trigeminal nerve; V2 – maxillary branch of the trigeminal nerve; V3 – mandibular branch of the trigeminal nerve; PVS – perivascular space; nasal-olf. art. – nasal olfactory artery; ACA – anterior cerebral artery; MCA – middle cerebral artery; Pcomm – posterior communicating artery; PCA – posterior cerebral artery. Coronal rat brain section schemas were adapted from the Rat brain atlas – 6th Edition (Paxinos and Watson, 2007) [40]. Approximate anteroposterior locations are provided relative to a coronal plane through bregma. Trigeminal nerves schema is adapted from [41]. Rat cerebral vasculature schema is adapted from [25]. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Cuts were made using Vannas scissors (Harvard Apparatus) at the left and right posterior communicating arteries under a stereo microscope (Olympus SZ61) ( ).

Techniques: Concentration Assay, Microscopy

Journal: STAR Protocols

Article Title: Protocol for in vivo and ex vivo assessments of glucose-stimulated insulin secretion in mouse islet β cells

doi: 10.1016/j.xpro.2021.100728

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Article Snippet: Vannas scissor , RWD life science , CAT# S11037-08.

Techniques: Recombinant, Suspension

Journal: STAR Protocols

Article Title: Protocol for high-resolution 3D visualization of insect regenerating legs through micro-computed tomography

doi: 10.1016/j.xpro.2024.103342

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Article Snippet: Vannas Spring scissors (8 cm) , RWD Life Science , Cat#S11001-08.

Techniques: Recombinant, Software, Micro-CT, Microscopy